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mouse nov ccn3 duoset elisa (R&D Systems)

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R&D Systems mouse nov ccn3 duoset elisa
Mouse Nov Ccn3 Duoset Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+nov+ccn3+duoset/Mouse+NOV%2FCCN3+DuoSet+ELISA/pmc12556222-62-0-5
Average 93 stars, based on 4 article reviews

mouse nov ccn3 duoset elisa - by Bioz Stars, 2026-10

93/100 stars

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Article Title: CCN3 Impairs Osteoblast and Stimulates Osteoclast Differentiation to Favor Breast Cancer Metastasis to Bone
Article Snippet: .. We used the mouse nov/ccn3 DuoSet (catalog number DY1976; R&D Systems, Minneapolis, MN) enzyme-linked immunosorbent assay (ELISA) kit to detect ccn3 in the conditioned medium harvested from the 4T1 derivatives and the human NOV/CCN3 DuoSet (catalog number DY1640; R&D Systems) ELISA kit from 66cl4 cells engineered to express human CCN3 . .. Conditioned media was diluted (1:2 for 4T1 derivatives and 1:10 for 66cl4 cells) into the reagent diluent concentrate 2 (catalog number DY995; R&D Systems).

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Fig. 1. CCN3 is expressed in anatomically distinct regions of the healthy adult murine CNS. Representative images of CCN3 and NeuN staining in CA1 field of hippocampus (A and B), subiculum (A and C), cerebral cortex (D–F), piriform cortex (G), suprachiasmatic nuclei (H), anterior olfactory nuclei (I), basomedial amygdala nuclei (J), thoracic spinal cord (K), cerebellum (L), and retina (M). (Scale bars: 100 μm [A; D; G; H; I, b; J; and K, a], 50 μm [M], 25 μm [B; C; E; F; I, c; and K, b], and 0.5 mm [I, a and L].) AON, anterior olfactory nuclei; BMA, basomedial amygdala nuclei; BrM, Bruch’s membrane; CA1, subfield 1 of hippocampus; CB, cerebellum; cc, corpus callosum; GM, gray matter; L, layer; MOB, main olfactory bulb; Pir, piriform cortex; SCN, suprachiasmatic nuclei; SUB, subiculum; WM, white matter.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Dynamic CCN3 expression in the murine CNS does not confer essential roles in myelination or remyelination.

doi: 10.1073/pnas.1922089117

Figure Lengend Snippet: Fig. 1. CCN3 is expressed in anatomically distinct regions of the healthy adult murine CNS. Representative images of CCN3 and NeuN staining in CA1 field of hippocampus (A and B), subiculum (A and C), cerebral cortex (D–F), piriform cortex (G), suprachiasmatic nuclei (H), anterior olfactory nuclei (I), basomedial amygdala nuclei (J), thoracic spinal cord (K), cerebellum (L), and retina (M). (Scale bars: 100 μm [A; D; G; H; I, b; J; and K, a], 50 μm [M], 25 μm [B; C; E; F; I, c; and K, b], and 0.5 mm [I, a and L].) AON, anterior olfactory nuclei; BMA, basomedial amygdala nuclei; BrM, Bruch’s membrane; CA1, subfield 1 of hippocampus; CB, cerebellum; cc, corpus callosum; GM, gray matter; L, layer; MOB, main olfactory bulb; Pir, piriform cortex; SCN, suprachiasmatic nuclei; SUB, subiculum; WM, white matter.

Article Snippet: Concentration of murine CCN3 in media from Treg and brain slice cultures was quantified by ELISA (DY1976, R&D Systems) according to manufacturer instructions.

Techniques: Staining, Membrane

Fig. 2. CCN3 is not required for myelination ex vivo. (A) CCN3 protein quantification via ELISA in cerebellar/brainstem slice conditioned media. Data are mean ± SD. Representative confocal projection images of MBP and NFH staining in brain slices at 7 (B) and 14 days ex vivo (dev) (D). (Scale bars: 25 μm.) Myelination index quantification (MBP+NFH+ colocalization area/NFH+ total area) of brain slices at 7 (C) and 14 dev (E). Data are mean ± SEM. ns, not sig- nificant. Statistical analysis: Mann–Whitney U test (C) or unpaired, two-tailed, Student’s t test (E). n = 3 wells containing 3 slices each (A) and 4–5 animals per group (C and E).

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Dynamic CCN3 expression in the murine CNS does not confer essential roles in myelination or remyelination.

doi: 10.1073/pnas.1922089117

Figure Lengend Snippet: Fig. 2. CCN3 is not required for myelination ex vivo. (A) CCN3 protein quantification via ELISA in cerebellar/brainstem slice conditioned media. Data are mean ± SD. Representative confocal projection images of MBP and NFH staining in brain slices at 7 (B) and 14 days ex vivo (dev) (D). (Scale bars: 25 μm.) Myelination index quantification (MBP+NFH+ colocalization area/NFH+ total area) of brain slices at 7 (C) and 14 dev (E). Data are mean ± SEM. ns, not sig- nificant. Statistical analysis: Mann–Whitney U test (C) or unpaired, two-tailed, Student’s t test (E). n = 3 wells containing 3 slices each (A) and 4–5 animals per group (C and E).

Article Snippet: Concentration of murine CCN3 in media from Treg and brain slice cultures was quantified by ELISA (DY1976, R&D Systems) according to manufacturer instructions.

Techniques: Ex Vivo, Enzyme-linked Immunosorbent Assay, Staining, MANN-WHITNEY, Two Tailed Test

Fig. 3. CCN3 is not required for CNS myelination in vivo. Representative images of Olig2 and CC1 staining in spinal cord vWM (A), medial corpus callosum (D), and motor cortex (G). OLC (B) and differentiated oligodendrocyte quantification (C) in spinal cord vWM. OLC (E) and differentiated oligodendrocyte quantification (F) in the medial corpus callosum. OLC (H) and differentiated oligodendrocyte quantification (I) in motor cortex. Representative images of Black Gold II myelin staining in medial corpus callosum (J) and motor cortex (K). (L) Representative images of toluidine blue staining of semithin spinal cord vWM sections. (M) Myelinated axon quantification in spinal cord vWM. (N) Representative images of NFH staining in spinal cord vWM. (O) Axonal quanti- fication in spinal cord vWM. (Scale bars: 100 μm [A, a; J; and K], 50 μm [G, a and D], and 25 μm [A, b; G, b; L; and N].) Data are mean ± SEM. ns, not significant. Statistical analysis: (B, C, E, F, H, I, M, O) unpaired, two-tailed, Student’s t tests. n = 4–6 (B, C, E, F, H, I, and O) and 3 (M) animals per group.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Dynamic CCN3 expression in the murine CNS does not confer essential roles in myelination or remyelination.

doi: 10.1073/pnas.1922089117

Figure Lengend Snippet: Fig. 3. CCN3 is not required for CNS myelination in vivo. Representative images of Olig2 and CC1 staining in spinal cord vWM (A), medial corpus callosum (D), and motor cortex (G). OLC (B) and differentiated oligodendrocyte quantification (C) in spinal cord vWM. OLC (E) and differentiated oligodendrocyte quantification (F) in the medial corpus callosum. OLC (H) and differentiated oligodendrocyte quantification (I) in motor cortex. Representative images of Black Gold II myelin staining in medial corpus callosum (J) and motor cortex (K). (L) Representative images of toluidine blue staining of semithin spinal cord vWM sections. (M) Myelinated axon quantification in spinal cord vWM. (N) Representative images of NFH staining in spinal cord vWM. (O) Axonal quanti- fication in spinal cord vWM. (Scale bars: 100 μm [A, a; J; and K], 50 μm [G, a and D], and 25 μm [A, b; G, b; L; and N].) Data are mean ± SEM. ns, not significant. Statistical analysis: (B, C, E, F, H, I, M, O) unpaired, two-tailed, Student’s t tests. n = 4–6 (B, C, E, F, H, I, and O) and 3 (M) animals per group.

Article Snippet: Concentration of murine CCN3 in media from Treg and brain slice cultures was quantified by ELISA (DY1976, R&D Systems) according to manufacturer instructions.

Techniques: In Vivo, Staining, Two Tailed Test

Fig. 4. CCN3 is transiently up-regulated in the spinal cord during remyelination. Representative images of CCN3 and Olig2 (A), CCN3, Olig2 and CC1 (F), and CCN3 and GFAP (K) staining in spinal cord of untreated controls, and 5 and 14 dpl after lysolecithin injection. (B) Representative confocal images of CCN3 and Olig2 staining in spinal cord lesions at 5 dpl. White arrowheads, CCN3+Olig2−cells; yellow arrowheads, CCN3+Olig2+ cells. CCN3+ cells (C), CCN3+Olig2+ cells (D), and percentage of CCN3+Olig2+ cells (E) quantification in untreated, 5 dpl, and 14 dpl spinal cord vWM. (G) Representative images of CCN3, Olig2, and CC1 staining in spinal cord lesions at 5 dpl. Yellow arrowheads, CCN3+Olig2+CC1+ cell. Proportion of OLCs that are CCN3+ (H), CCN3+Olig2+CC1+ cells (I) and proportion of CCN3-expressing OLCs that are CC1+ (J) quantification in untreated, 5 dpl, and 14 dpl spinal cord vWM. (L) Representative confocal images of CCN3 and GFAP staining in spinal cord lesions at 5 dpl. White arrowheads, CCN3+GFAP−cells; yellow arrowheads, CCN3+GFAP+ cells. CCN3+ cells (M), CCN3+GFAP+ cells (N), and percentage of CCN3+GFAP+ cell quantification (O) in untreated, 5 dpl, and 14 dpl spinal cord vWM. (Scale bars: F and K, 100 μm; A, 50 μm; B, G, and L, 25 μm.) Data are mean ± SEM. ns, not significant; *P < 0.05; **P < 0.01. Statistical analysis: Kruskal–Wallis with Dunn’s Multiple Comparison test. n = 4–10 animals per group.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Dynamic CCN3 expression in the murine CNS does not confer essential roles in myelination or remyelination.

doi: 10.1073/pnas.1922089117

Figure Lengend Snippet: Fig. 4. CCN3 is transiently up-regulated in the spinal cord during remyelination. Representative images of CCN3 and Olig2 (A), CCN3, Olig2 and CC1 (F), and CCN3 and GFAP (K) staining in spinal cord of untreated controls, and 5 and 14 dpl after lysolecithin injection. (B) Representative confocal images of CCN3 and Olig2 staining in spinal cord lesions at 5 dpl. White arrowheads, CCN3+Olig2−cells; yellow arrowheads, CCN3+Olig2+ cells. CCN3+ cells (C), CCN3+Olig2+ cells (D), and percentage of CCN3+Olig2+ cells (E) quantification in untreated, 5 dpl, and 14 dpl spinal cord vWM. (G) Representative images of CCN3, Olig2, and CC1 staining in spinal cord lesions at 5 dpl. Yellow arrowheads, CCN3+Olig2+CC1+ cell. Proportion of OLCs that are CCN3+ (H), CCN3+Olig2+CC1+ cells (I) and proportion of CCN3-expressing OLCs that are CC1+ (J) quantification in untreated, 5 dpl, and 14 dpl spinal cord vWM. (L) Representative confocal images of CCN3 and GFAP staining in spinal cord lesions at 5 dpl. White arrowheads, CCN3+GFAP−cells; yellow arrowheads, CCN3+GFAP+ cells. CCN3+ cells (M), CCN3+GFAP+ cells (N), and percentage of CCN3+GFAP+ cell quantification (O) in untreated, 5 dpl, and 14 dpl spinal cord vWM. (Scale bars: F and K, 100 μm; A, 50 μm; B, G, and L, 25 μm.) Data are mean ± SEM. ns, not significant; *P < 0.05; **P < 0.01. Statistical analysis: Kruskal–Wallis with Dunn’s Multiple Comparison test. n = 4–10 animals per group.

Article Snippet: Concentration of murine CCN3 in media from Treg and brain slice cultures was quantified by ELISA (DY1976, R&D Systems) according to manufacturer instructions.

Techniques: Staining, Injection, Expressing, Comparison

Fig. 5. CCN3 is transiently up-regulated in the lateral septum and corpus callosum during demyelination. (A) Representative images of CCN3 staining in septal nuclei and corpus callosum in untreated controls, cuprizone-induced demyelination, and remyelination. cc, corpus callosum; ls, lateral septum; lv, lateral ventricle. (B) Representative images of CCN3 staining in the corpus callosum and lateral septum 4 wk after cuprizone-induced demyelination. (C) Percentage of CCN3+ area quantification in septal nuclei of untreated, cuprizone-fed and cuprizone-withdrawn mice. (D) Representative confocal images of CCN3, Olig2, and CC1 staining in the medial corpus callosum of untreated controls and WT mice fed with cuprizone for 4 wk. (E) Representative confocal images of CCN3, NF200, and APP staining in the lateral septum and corpus callosum 4 wk after cuprizone-induced demyelination. (Scale bars: A, 200 μm; B, 50 μm; D and E, 25 μm; E, a and b, 10 μm.) Data are mean ± SEM. ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001. Statistical analysis: Kruskal–Wallis with Dunn’s Multiple Comparison test. n = 4–6 animals per group.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Dynamic CCN3 expression in the murine CNS does not confer essential roles in myelination or remyelination.

doi: 10.1073/pnas.1922089117

Figure Lengend Snippet: Fig. 5. CCN3 is transiently up-regulated in the lateral septum and corpus callosum during demyelination. (A) Representative images of CCN3 staining in septal nuclei and corpus callosum in untreated controls, cuprizone-induced demyelination, and remyelination. cc, corpus callosum; ls, lateral septum; lv, lateral ventricle. (B) Representative images of CCN3 staining in the corpus callosum and lateral septum 4 wk after cuprizone-induced demyelination. (C) Percentage of CCN3+ area quantification in septal nuclei of untreated, cuprizone-fed and cuprizone-withdrawn mice. (D) Representative confocal images of CCN3, Olig2, and CC1 staining in the medial corpus callosum of untreated controls and WT mice fed with cuprizone for 4 wk. (E) Representative confocal images of CCN3, NF200, and APP staining in the lateral septum and corpus callosum 4 wk after cuprizone-induced demyelination. (Scale bars: A, 200 μm; B, 50 μm; D and E, 25 μm; E, a and b, 10 μm.) Data are mean ± SEM. ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001. Statistical analysis: Kruskal–Wallis with Dunn’s Multiple Comparison test. n = 4–6 animals per group.

Article Snippet: Concentration of murine CCN3 in media from Treg and brain slice cultures was quantified by ELISA (DY1976, R&D Systems) according to manufacturer instructions.

Techniques: Staining, Comparison

Fig. 6. CCN3 is not required for OPC proliferation or differentiation during spinal cord remyelination. Representative images of Iba1 and MBP (A and B) and Olig2 and Ki67 (C and D) staining 5 dpl after lysolecithin injection in spinal cord vWM. White arrowheads, Olig2+Ki67+ cells. OLC (E), proliferating OPC (F), and percentage of proliferating OPC quantification (G) at 5 dpl. Representative images of Black Gold II (H) and Olig2 and CC1 (I and J) staining at 14 dpl. White arrowheads, Olig2+CC1+ cells. OLC (K), differentiated oligodendrocyte (L), and percentage of differentiated OPC quantification (M) at 14 dpl. (Scale bars: A, C, and I, 100 μm; H, 50 μm; B, D, and J, 25 μm.) Data are mean ± SEM. ns, not significant. Statistical analysis: unpaired, two-tailed, Student’s t tests (E, F, K, and L) or Mann–Whitney U tests (G and M). n = 4–5 (E–G) and 11 (K–M) animals per group.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Dynamic CCN3 expression in the murine CNS does not confer essential roles in myelination or remyelination.

doi: 10.1073/pnas.1922089117

Figure Lengend Snippet: Fig. 6. CCN3 is not required for OPC proliferation or differentiation during spinal cord remyelination. Representative images of Iba1 and MBP (A and B) and Olig2 and Ki67 (C and D) staining 5 dpl after lysolecithin injection in spinal cord vWM. White arrowheads, Olig2+Ki67+ cells. OLC (E), proliferating OPC (F), and percentage of proliferating OPC quantification (G) at 5 dpl. Representative images of Black Gold II (H) and Olig2 and CC1 (I and J) staining at 14 dpl. White arrowheads, Olig2+CC1+ cells. OLC (K), differentiated oligodendrocyte (L), and percentage of differentiated OPC quantification (M) at 14 dpl. (Scale bars: A, C, and I, 100 μm; H, 50 μm; B, D, and J, 25 μm.) Data are mean ± SEM. ns, not significant. Statistical analysis: unpaired, two-tailed, Student’s t tests (E, F, K, and L) or Mann–Whitney U tests (G and M). n = 4–5 (E–G) and 11 (K–M) animals per group.

Article Snippet: Concentration of murine CCN3 in media from Treg and brain slice cultures was quantified by ELISA (DY1976, R&D Systems) according to manufacturer instructions.

Techniques: Staining, Injection, Two Tailed Test, MANN-WHITNEY

Fig. 7. CCN3 is not required for OPC differentiation during remyelination in the brain. (A) Representative images of Black Gold II myelin staining in medial corpus callosum of untreated, 6 wk demyelinated and 2 wk remyelinating WT and CCN3−/−mice. (B) Representative images of Olig2 and CC1 staining in corpus callosum of untreated, demyelinated and remyelinating mice. (Scale bars: 100 μm.) OLC (C) and differentiated OPC quantification (D) in medial corpus callosum. Data are mean ± SEM. ns, not significant. Statistical analysis: (C and D) Kruskal–Wallis with Dunn’s Multiple Comparison test. n = 3–6 animals per group.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Dynamic CCN3 expression in the murine CNS does not confer essential roles in myelination or remyelination.

doi: 10.1073/pnas.1922089117

Figure Lengend Snippet: Fig. 7. CCN3 is not required for OPC differentiation during remyelination in the brain. (A) Representative images of Black Gold II myelin staining in medial corpus callosum of untreated, 6 wk demyelinated and 2 wk remyelinating WT and CCN3−/−mice. (B) Representative images of Olig2 and CC1 staining in corpus callosum of untreated, demyelinated and remyelinating mice. (Scale bars: 100 μm.) OLC (C) and differentiated OPC quantification (D) in medial corpus callosum. Data are mean ± SEM. ns, not significant. Statistical analysis: (C and D) Kruskal–Wallis with Dunn’s Multiple Comparison test. n = 3–6 animals per group.

Article Snippet: Concentration of murine CCN3 in media from Treg and brain slice cultures was quantified by ELISA (DY1976, R&D Systems) according to manufacturer instructions.

Techniques: Staining, Comparison

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